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  • Data from "KDM5B decommissions the H3K4 methylation landscape of self-renewal genes during trophoblast stem cell differentiation"
    WSU Dataset

    Authors
    Jian Xu
    Benjamin L. Kidder
    Description

    Trophoblast stem (TS) cells derived from the trophectoderm (TE) of mammalian embryos have the ability to self-renew indefinitely or differentiate into fetal lineages of the placenta. Epigenetic control of gene expression plays an instrumental role in dictating the fate of TS cell self-renewal and differentiation. However, the roles of histone demethylases and activating histone modifications such as methylation of histone 3 lysine 4 (H3K4me3/me2) in regulating TS cell expression programs, and in priming the epigenetic landscape for trophoblast differentiation, are largely unknown. This study demonstrates that the H3K4 demethylase, KDM5B, regulates the H3K4 methylome and expression landscapes of TS cells. Depletion of KDM5B resulted in downregulation of TS cell self-renewal genes and upregulation of trophoblast-lineage genes, which was accompanied by altered H3K4 methylation. Moreover, it is found that KDM5B resets the H3K4 methylation landscape during differentiation in the absence of the external self-renewal signal, FGF4, by removing H3K4 methylation from promoters of self-renewal genes, and of genes whose expression is enriched in TS cells. Altogether, these data indicate an epigenetic role for KDM5B in regulating H3K4 methylation in TS cells and during trophoblast differentiation.

    Subject
    Biology
    Access Rights
    Free to all
  • Glass promotes the differentiation of both neuronal and non-neuronal cells in the Drosophila eye
    WSU Dataset

    Authors
    Carolyn A. Morrison
    Hao Chen
    Tiffany Cook
    Stuart Brown
    1 more author(s)...
    Description

    Multiple cell types can be specified from a single pool of progenitors through the combinatorial activity of transcriptional regulators, which activate distinct developmental programs to establish different cell fates. The zinc finger transcription factor Glass is required for neuronal progenitors in the Drosophila eye imaginal disc to acquire a photoreceptor identity. Glass is also expressed in non-neuronal cone and pigment cells, but its role in these cells is unknown. To examine how Glass activity is affected by the cellular context, the researchers misexpressed it in different tissues. When expressed in neuroblasts of the larval brain or in epithelial cells of the wing disc, Glass activated both a common core set of target genes and distinct gene sets specific to each tissue. In addition to photoreceptor-specific genes, Glass induced markers of cone and pigment cells. Cell type-specific glass mutations generated in cone or pigment cells using somatic CRISPR revealed autonomous developmental defects, and expressing Glass specifically in these cells partially rescued glass mutant phenotypes. Glass thus acts in both neuronal and non-neuronal cells to promote their differentiation into functional components of the eye, suggesting that it is a determinant of organ identity.

    Subject
    Biology
    Access Rights
    Free to all
  • Data from "Contribution of H3K4 demethylase KDM5B to nucleosome organization in embryonic stem cells revealed by micrococcal nuclease sequencing"
    WSU Dataset

    Authors
    Jiji T. Kurup
    Ion J. Campeanu
    Benjamin L. Kidder
    Description

    Positioning of nucleosomes along DNA is an integral regulator of chromatin accessibility and gene expression in diverse cell types. However, the precise nature of how histone demethylases including the histone 3 lysine 4 (H3K4) demethylase, KDM5B, impacts nucleosome positioning around transcriptional start sites (TSS) of active genes is poorly understood. Therefore, to clarify the role for KDM5B in regulating nucleosome organization in ES cells, this study evaluated genome-wide changes in nucleosome positioning in KDM5B-depleted and control ES cells using micrococcal nuclease sequencing (MNase-Seq). These findings demonstrate that depletion of KDM5B leads to altered enrichment of nucleosomes around TSS regions and accessible chromatin regions (DNase I hypersensitive sites).

    Subject
    Biology
    Access Rights
    Free to all
  • Data from "Characterization of retinal regeneration in adult zebrafish following multiple rounds of phototoxic lesion"
    WSU Dataset

    Authors
    Alexandra H. Ranski
    Ashley C. Kramer
    Gregory W. Morgan
    Jennifer L. Perez
    1 more author(s)...
    Description

    Müller glia in the zebrafish retina respond to retinal damage by re-entering the cell cycle, which generates large numbers of retinal progenitors that ultimately replace the lost neurons. In this study, researchers compared the regenerative outcomes of adult zebrafish exposed to one round of phototoxic treatment with adult zebrafish exposed to six consecutive rounds of phototoxic treatment. It was observed that Müller glia continued to re-enter the cell cycle to produce clusters of retinal progenitors in zebrafish exposed to multiple rounds of phototoxic light.

    Subject
    Biology
    Access Rights
    Free to all
  • Data from "Multifunctional glial support by Semper cells in the Drosophila retina"
    WSU Dataset

    Authors
    Mark A. Charlton-Perkins
    Edward D. Sendler
    Elke K. Buschbeck
    Tiffany Cook
    Description

    Glial cells play structural and functional roles central to the formation, activity and integrity of neurons throughout the nervous system. Here, using the genetic model Drosophila melanogaster, reseearchers identify a new glial cell type in one of the most active tissues in the nervous system—the retina. These cells, called ommatidial cone cells (or Semper cells), were previously recognized for their role in lens formation. Using cell-specific molecular genetic approaches, this study demonstrates that cone cells (CCs) also share molecular, functional, and genetic features with both vertebrate and invertebrate glia to prevent light-induced retinal degeneration and provide structural and physiological support for photoreceptors.

    Subject
    Biology
    Access Rights
    Free to all
  • Global signaling profiling in a human model of tumorigenic progression indicates a role for alternative RNA splicing in cellular reprogramming
    WSU Dataset

    Authors
    Joseph A. Caruso
    Nicholas Carruthers
    Bryan Thibodeau
    Timothy J. Geddes
    2 more author(s)...
    Description

    Phosophoproteomic analysis was used to profile cell lines in the MCF-10A lineage of human mammary epithelial cells to determine how human breast cells can be reprogrammed during tumorigenic progression. Data were collected using a LTQ-XL mass spectrometer (Thermo). Phosphopeptides were enriched from cell extracts from 3 independent biological replicates, and each replicate was analyzed as 3 technical replicates for a total of 9 LC/MS/MS runs per cell line.

    Subject
    Medicine & Health
    Access Rights
    Free to all
  • Wayne State University Dashboard
    WSU Dataset

    Description

    Interactive visualizations of Wayne State University data on student enrollment (by college, class level, full/part time status, race/ethnicity, and gender), degree completion (by college/department/major, race/ethnicity, STEM vs. non-STEM), faculty and staff count (by college, EEO category, tenure status, race/ethnicity, and gender), finance (budget by revenue source, account pool group, program, and college) research (number of awards and total dollar amount by sponsor), and development (gifts recorded and pledges received).

    Subject
    Education
    Geographic Coverage
    Detroit, Michigan
    Timeframe
    2004 - Present
    Access Rights
    Free to all
    Local Expert
    Song Yan
  • Data from "Long-term safety of rituximab induced peripheral B-cell depletion in autoimmune neurological diseases"
    WSU Dataset

    Authors
    Anza B. Memon
    Adil Javed
    Christina Caon
    Shitiz Srivastawa
    6 more author(s)...
    Description

    B-cells play a pivotal role in several autoimmune diseases, including patients with immune-mediated neurological disorders (PIMND), such as neuromyelitis optica (NMO), multiple sclerosis (MS), and myasthenia gravis (MG). Targeting B-cells has been an effective approach in ameliorating both central and peripheral autoimmune diseases. However, there is a paucity of literature on the safety of continuous B-cell depletion over a long period of time. The aim of this study was to examine the long-term safety, incidence of infections, and malignancies in subjects receiving continuous therapy with a B-cell depleting agent rituximab over at least 3 years or longer. This was a retrospective study involving PIMND who received continuous cycles of rituximab infusions every 6 to 9 months for up to 7 years. The incidence of infection related adverse events (AE), serious adverse events (SAE), and malignancies were observed.

    Subject
    Biology
    Medicine & Health
    Access Rights
    Free to all
  • Data from "Mercury alters endogenous phosphorylation profiles of SYK in murine B cells"
    WSU Dataset

    Authors
    Joseph A. Caruso
    Nicholas Carruthers
    Namhee Shin
    Randal Gill
    2 more author(s)...
    Description

    Proteomics data from a study on whether mercury exposure alters B cell responsiveness to self-antigens by interfering with B cell receptor (BCR) signal transduction. These data show the effects of mercury on the protein tyrosine kinase SYK, a critical protein involved in regulation of the BCR signaling pathway. The raw data for quantitation of SYK phosphorylation status of selected sites were obtained using multiple reaction monitoring (MRM) on a TSQ triple quadrupole mass spectrometer.

    Subject
    Biology
    Timeframe
    2017 - 2017
    Access Rights
    Free to all

 

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