Data Catalog
Search here to find large public and licensed datasets
Data from "Contribution of H3K4 demethylase KDM5B to nucleosome organization in embryonic stem cells revealed by micrococcal nuclease sequencing" WSU Dataset
UID: 102
- Description
- Positioning of nucleosomes along DNA is an integral regulator of chromatin accessibility and gene expression in diverse cell types. However, the precise nature of how histone demethylases including the histone 3 lysine 4 (H3K4) demethylase, KDM5B, impacts nucleosome positioning around transcriptional start sites (TSS) of active genes is poorly understood. Therefore, to clarify the role for KDM5B in regulating nucleosome organization in ES cells, this study evaluated genome-wide changes in nucleosome positioning in KDM5B-depleted and control ES cells using micrococcal nuclease sequencing (MNase-Seq). These findings demonstrate that depletion of KDM5B leads to altered enrichment of nucleosomes around TSS regions and accessible chromatin regions (DNase I hypersensitive sites).
-
Access via GEO
Accession #: GSE123249 - Access Restrictions
-
Free to all
- Access Instructions
- Data can be accessed by clicking the above link.
- Associated Publications
- Equipment Used
-
Illumina HiSeq 4000
- Dataset Format(s)
- WIG
- Dataset Size
- 157.3 MB
Do you have or know of a dataset that should be added to the catalog? Let us know!